UMass Light Microscopy currently has the following microscopes available for use

A1R: Nikon A1 Resonant Scanning Confocal with TIRF module (Morrill) (view-only calendar)

  • Objectives: 10x, 20x, 40x, 60x, 100x, 60x TIRF, 100x TIRF
  • Laser lines: 405, 435, 488, 514, 561, 640 nm
  • Detectors: 5 detectors: 1 transmitted light, 2 high-sensitivity PMTs, and 2 GaAsP detectors
  • Camera: Andor Xyla
  • This microscope is very versatile and can be used for live or fixed samples.  The resonant scanner allows for very fast acquisitions and the GaAsP detectors are extremely sensitive.  Read more about this microscope at Nikon’s site.

A1R-SIMe: Nikon A1 Resonant Scanning Confocal with Structured Illumination (view-only calendar)

  • Objectives: 10x, 20x, 40x, 60x, 100x, 60x dry
  • Laser lines: 405, 488, 561, 640 nm for confocal; 488, 561, 640 nm for SIM
  • Detectors: 5 detectors: 1 transmitted light, 2 high-sensitivity PMTs, and 2 GaAsP detectors; sCMOS for SIM
  • This microscope is very versatile and can be used for live or fixed samples.  The resonant scanner allows for very fast acquisitions and the GaAsP detectors are extremely sensitive.  The SIM side is extremely easy to use with no special sample preparation required for super-resolution imaging. Read more about this microscope at Nikon’s site.

SD: Nikon with Yokogawa Spinning Disk Confocal (view-only calendar)

  • Objectives: 20x, 40x water immersion, 40x oil immersion, 60x, 100x
  • Laser lines: 405, 488, 561, 640 nm for imaging and 405, 488, 561, 640 nm for photoactivation/bleaching/etc.
  • Detectors: Andor EMCCD camera
  • Stage: Piezo
  • This microscope is great for live cells as it is a low-light technique.  With four laser lines and an additional mini-scanner for PA/FRAP/etc., we can easily image dynamic movements in live cells.

A1SP: Nikon A1 Spectral Detector Confocal (view-only calendar)

  • Objectives: 10x, 20x, 40x, 60x, 100x
  • Laser lines: 405, 488, 561, 640 nm
  • Detectors: 5 detectors: 1 transmitted light, two high-sensitivity PMTs, and 2 GaAsP detectors
  • This microscope is great for fixed samples and is especially useful when experimenters may have overlapping emissions from fluorophores or autofluorescence.  The 32-channel spectral detector can be implemented with the click of a button and allows for 32 x 2.5 – 10 nm bins of fluorescence identification.  Read more about this microscope at Nikon’s site.

A1MP: Nikon A1 Resonant Scanning Multi-Photon Confocal (view-only calendar)

  • Objectives: 25x extremely long working distance (upright)
  • Laser lines: 760-1040 nm
  • Detectors: 1 high-sensitivity PMT, 3 GaAsP detectors
  • This microscope is an upright, manual microscope that is suited for in vivo, intravital imaging as well as imaging in and through thick tissues and samples.  It uses a tunable infrared pulsed laser to excite fluorophores at the focal volume and features a resonance scanner that can image very quickly along with a fast moving piezo stage.

HCA: Nikon with High Content Analysis (view-only calendar)

  • Objectives: All “air” objectives; 10x, 20x, 20xELWD, 40x, 40xELWD, 60x
  • Excitation colors: 395, 415, 445, 488, 515, 540, 590, 640
  • Detectors: Widefield with an Andor Zyla sCMOS camera and also a Crest spinning disk confocal with a ProEM camera.
  • This microscope is truly amazing for its ability to collect and automatically analyze data from live or fixed samples.  The intuitive and adaptive software can be programed to count cells, monitor growth, take high-resolution pictures when a certain feature is found, scan slides, scan multi-well plates, etc.  When it is done, you can have the microscope send you a text message that contains any key variables that you need to know.

N-STORM: Nikon STORM (view-only calendar)

  • Objectives: 20X, 60x TIRF, 100 TIRF (HP)
  • Laser lines: 405, 488, 561, 640
  • Detectors: Hamamatsu ORCA-Flash4.0
  • This microscope makes doing 3D STORM imaging straightforward.  This has STORM-4.0 which includes a cylindrical lens to provide z-information on your molecules of interest.  This also has the option to change not only the TIRF angle with the click of a button, but also the direction of the laser entering the back aperture of the objective.

LCMD: Nikon with Arcturus Laser Capture Micro-Dissection (view-only calendar)

  • Objectives: 4x, 10x, 40x
  • Laser lines: UV, IR
  • Detectors: Color camera
  • This microscope is really a cellular robot.  You can find cells or regions that you are interested on tissue slices and draw a line around them, cut them out, move them to a cap and then process the cap for downstream experiments (sequencing, proteomics, etc.)  The interface is very easy to use and you can read more about it at the manufacturer’s site.

Workstations

Incubators

  • Tokai Hit on-stage incubator with CO2, heat, and humidity
  • Oko Lab on-stage incubator with CO2, heat, and humidity
  • 4 full size incubators available for use (one has O2 control)

Coming soon; 1 other research microscope, 1 widefield microscope, and a FLIM accessory.